{"id":190,"date":"2017-11-27T20:24:46","date_gmt":"2017-11-27T22:24:46","guid":{"rendered":"http:\/\/sites.usp.br\/land\/?page_id=190"},"modified":"2023-10-04T12:40:14","modified_gmt":"2023-10-04T14:40:14","slug":"linhasdepesquisa","status":"publish","type":"page","link":"https:\/\/land.icb.usp.br\/en\/linhasdepesquisa\/","title":{"rendered":"Lines of research"},"content":{"rendered":"

Contribution to Science<\/b><\/span><\/p>\n

1. Effect of photobiomodulation on different peripheral neuropathies in rats:<\/b>\u00a0 The technique of photobiomodulation (PBMT), use of light as therapy, is a non-invasive method that has been shown both in basic and in clinical research to be effective in reducing pain sensitivity and consequently improving the quality of life of patients with neuropathic pain. Our group demonstrated the effects of this therapy on pain sensitivity and sciatic nerve repair induced by peripheral neuropathies such as chronic constrictive injury (CCI) and type 1 diabetes mellitus in rats, using in vitro assays and behavioral analyses. Our ongoing and published results point to an improvement in the nociceptive response in the PBMT group when compared to the untreated (sham treated) group in both experimental models, suggesting that PBMT treatment improves nociception induced by neuropathic lesions induces an improvement in sciatic nerve regeneration and decrease pro-inflammatory cytokines. Furthermore, studies demonstrate that near-infrared light penetrates in the tissue where it is absorbed by chromophores, resulting in an increase in cellular metabolism through the increase of ATP synthesis by mitochondria. With this, our main objective is to better understand the mechanisms involved in photobiomodulation as a new strategy for personalized pain therapy.<\/span><\/span><\/p>\n

2. Neonatal Anoxia and sex differences:<\/b> Neonatal anoxia is a well-known world health problem that results in neurodevelopmental deficits, such as sensory alterations that are observed in patients with cerebral palsy and autism disorder, for which oxygen deprivation is a risk factor. Nociceptive responses, as part of the pain sensory system, have been reported to be altered in these patients. We are investigating whether oxygen deprivation alters nociceptive sensitivity in rats and mice, male and female. Our results to date, in collaboration with Dr Nogueira, M.I. and my Laboratory, suggests a negative impact of neonatal anoxia on pain responsivity. Our data indicate that depriving the nervous system of oxygen may affect the normal functioning of sensorimotor systems, including exhibiting sex differences in the results obtained.<\/span><\/span><\/p>\n

3. Modulation of the cannabinoid system and toxic aldehydes as a therapeutic approach for experimental multiple sclerosis:<\/b> Multiple sclerosis is a neurodegenerative disease that affects the central nervous system, inducing the activation of immune system and inflammation, both in the periphery and central nervous system. It is a disorder in which the immune system recognizes components of the CNS as foreign, compromising the function of neurons, leading to motor, cognitive, and sensory changes. This project aims to evaluate the effect of crotalfine, an analgesic peptide with action on CB2, and Alda-1, a small molecule that decreases the production of toxic aldehydes, on motor and nociceptive changes in mice with experimental autoimmune encephalomyelitis. Our results demonstrated that myelin loss occurs in the periphery and not just in the CNS. Our treatments proved able of reverse demyelination, enhancing recovery of motor function. Thus, our project brings new perspectives for the treatment of multiple sclerosis, and potentially other neurodegenerative diseases. This work it is a collaborative research between Institute Butantan and Institute of Biomedical Science, both in Sao Paulo, Brazil.<\/span><\/span><\/p>\n

4. Role of opioids, cannabinoids and neuroinflammation systems on pain in rats with Parkinson Disease (PD): <\/b>Currently, many clinical studies and animal models emphasize the beneficial effects promoted by physical exercise in PD with a focus on motor symptoms, leaving aside the cellular and molecular events that may be correlated with the improvement of chronic pain after this non-pharmacological intervention. Physiotherapy and physical training have been shown to be efficient for improving the quality of life of patients diagnosed with PD who report pain. Although previous studies in PD patients describe a correlation between pain threshold and activation of different areas in brain. Little is known about the mechanisms involved in improving pain promoted by physical exercise in PD described in clinical studies. Thus, the present collaborative study becomes important for evaluating areas of the CNS, in addition to the pathway, areas that may be modulated by physical exercise on a treadmill, which may be directly or indirectly be involved in nociceptive processing in the animal model of PD, such as PAG and anterior cingulate cortex. This is a collaborative work between Institute of Biomedical Science and Institute of Clinical Medicine, Aarhus University, Denmark with Dr Professor Anne M. Landau.<\/span><\/span><\/p>\n

5. Transcranial Photobiomodulation as Therapy for Parkinson’s Disease Patients: Relationship Between Pain and Brain Functional Connectivity:<\/b><\/span><\/p>\n

Parkinson disease is a progressive and degenerative neurological movement disorder that affects thousands of people. The disease is characterized by presenting motor and non-motor symptoms, as the disease progresses, it becomes more disabling, making it impossible for the individual to perform simple tasks. A non-motor symptom increasingly reported by patients and undertreated in clinical practice is pain. During the past few decades, possible neural substrates of pain have been studied extensively, resulting in a potential network of connected brain areas that are believed to underlie pain processing and experience. There is no definitive consensus on all areas involved in such a pain network; however, pain-related regions consistently found across all studies include the thalamus, anterior cingulate cortex (ACC), posterior and anterior insula, amygdala, prefrontal cortex (PFC), secondary somatosensory cortex (IBS), and periaqueductal gray (PAG). With the aim of helping to improve the painful condition, non-pharmacological therapies have been studied, and one of them is phototherapy, a non-invasive method used by several areas of health, which has been shown to be increasingly effective in the treatment of decreased pain sensitivity. The present study aims to evaluate the effects of transcranial photobiomodulation in patients with Parkinson\u2019s disease. This is a randomized study, in which we intend to analyze the effect of FBM on pain control and on magnetic resonance images.\u00a0 This is a collaborative work with Institute of Biomedical Science, Universidade de Maryland; Dr Joyce Teixeira, InRad- FMUSP; Dra. Maria da Gra\u00e7a Martin and Prof Dr. Hazem Adel Ashmawi.<\/span><\/p>\n

6. Association of photobiomodulation and swimming excercise in rats with neuropathic\u00a0 pain<\/b>: In clinical practice, it has been widely reported that neuropathic pain is difficult to treat, in addition, neuropathic pain has been reported as an important public health issue. Therapeutic options for the management of neuropathic pain have been increased along the years, however the response of these patients is still not satisfactory. In the last several studies have shown that non-pharmacologic treatments such as photobiomodulation and swimming have been increasingly used as adjuncts to clinical practice. The aim of this work is to evaluate whether swimming associated or not with photobiomodulation has its effects potenciallized on a model of chronic constriction injury. An investigation of therapeutic alternatives for the treatment of neuropathic pain is important, since this pathology does not respond satisfactorily to any type of conventional intervention. Our results have demonstrated a beneficial effect on pain and motor response when we associate both therapies.<\/span><\/p>\n

\n

1. Efeito da fotobiomodula\u00e7\u00e3o em diferentes neuropatias perif\u00e9ricas em ratos<\/b>: A t\u00e9cnica da fotobiomodula\u00e7\u00e3o (PBMT), uso da luz como terapia, \u00e9 um m\u00e9todo n\u00e3o invasivo que tem demonstrado ser eficaz na redu\u00e7\u00e3o da sensibilidade \u00e0 dor e, consequentemente, na melhoria da qualidade de vida de pacientes com dor neurop\u00e1tica, tanto em pesquisas b\u00e1sicas quanto cl\u00ednicas. Nosso grupo demonstrou os efeitos dessa terapia na sensibilidade \u00e0 dor e na repara\u00e7\u00e3o do nervo ci\u00e1tico induzida por neuropatias perif\u00e9ricas, como les\u00e3o constritiva cr\u00f4nica (CCI) e diabetes mellitus tipo 1 em ratos, usando ensaios in vitro e an\u00e1lises comportamentais. Nossos resultados em andamento e publicados indicam uma melhoria na resposta nociceptiva no grupo de PBMT em compara\u00e7\u00e3o com o grupo n\u00e3o tratado em ambos os modelos experimentais, sugerindo que o tratamento com PBMT melhora a nocicep\u00e7\u00e3o induzida por les\u00f5es neurop\u00e1ticas, induz uma melhoria na regenera\u00e7\u00e3o do nervo ci\u00e1tico e diminui citocinas pr\u00f3-inflamat\u00f3rias. Al\u00e9m disso, estudos demonstram que a luz infravermelha pr\u00f3xima penetra nos tecidos onde \u00e9 absorvida por crom\u00f3foros, resultando em um aumento no metabolismo celular por meio do aumento da s\u00edntese de ATP pelas mitoc\u00f4ndrias. Com isso, nosso principal objetivo \u00e9 compreender melhor os mecanismos envolvidos na fotobiomodula\u00e7\u00e3o como uma nova estrat\u00e9gia para a terapia personalizada da dor.<\/span><\/p>\n

2. Anoxia neonatal e diferen\u00e7as de g\u00eanero:<\/b> A anoxia neonatal \u00e9 um problema de sa\u00fade mundial bem conhecido que resulta em d\u00e9ficits no neurodesenvolvimento, como altera\u00e7\u00f5es sensoriais observadas em pacientes com paralisia cerebral e transtorno do espectro autista, para os quais a priva\u00e7\u00e3o de oxig\u00eanio \u00e9 um fator de risco. Respostas nociceptivas, como parte do sistema sensorial da dor, foram relatadas como alteradas nesses pacientes. Estamos investigando se a priva\u00e7\u00e3o de oxig\u00eanio altera a sensibilidade nociceptiva em ratos e camundongos, machos e f\u00eameas. Nossos resultados at\u00e9 o momento, em colabora\u00e7\u00e3o com a Dra. Nogueira, M.I. e meu laborat\u00f3rio, sugerem um impacto negativo da anoxia neonatal na responsividade \u00e0 dor. Nossos dados indicam que privar o sistema nervoso<\/span> de <\/span>oxig\u00eanio pode afetar o funcionamento normal dos sistemas sensorimotores, incluindo a manifesta\u00e7\u00e3o de diferen\u00e7as de g\u00eanero nos resultados obtidos.<\/span><\/span><\/p>\n

3. <\/b>Modula\u00e7\u00e3o do sistema canabinoide e alde\u00eddos t\u00f3xicos como abordagem terap\u00eautica para esclerose m\u00faltipla experimental:<\/b> A esclerose m\u00faltipla \u00e9 uma doen\u00e7a neurodegenerativa que afeta o sistema nervoso central, induzindo a ativa\u00e7\u00e3o do sistema imunol\u00f3gico e a inflama\u00e7\u00e3o, tanto na periferia quanto no sistema nervoso central. \u00c9 uma desordem na qual o sistema imunol\u00f3gico reconhece componentes do SNC como estranhos, comprometendo a fun\u00e7\u00e3o dos neur\u00f4nios e levando a altera\u00e7\u00f5es motoras, cognitivas e sensoriais. Este projeto tem como objetivo avaliar o efeito da crotalfina, um pept\u00eddeo analg\u00e9sico com a\u00e7\u00e3o sobre o CB2, e da Alda-1, uma pequena mol\u00e9cula que diminui a produ\u00e7\u00e3o de alde\u00eddos t\u00f3xicos, nas altera\u00e7\u00f5es motoras e nociceptivas em camundongos com encefalomielite autoimune experimental. Nossos resultados demonstraram que a perda de mielina ocorre na periferia e n\u00e3o apenas no SNC. Nossos<\/span> tratamentos provaram ser capazes de reverter a desmieliniza\u00e7\u00e3o, melhorando a recupera\u00e7\u00e3o da fun\u00e7\u00e3o motora. Assim, nosso projeto traz novas perspectivas para o tratamento da esclerose m\u00faltipla e, potencialmente, de outras doen\u00e7as neurodegenerativas. Este trabalho \u00e9 uma pesquisa colaborativa entre o Instituto Butantan e o Instituto de Ci\u00eancias Biom\u00e9dicas, ambos em S\u00e3o Paulo, Brasil.<\/span><\/span><\/p>\n

4. <\/b>Papel dos opioides, canabinoides e sistemas de neuroinflama\u00e7\u00e3o na dor em ratos com doen\u00e7a de Parkinson (DP):<\/b> Atualmente, muitos estudos cl\u00ednicos e modelos animais enfatizam os efeitos ben\u00e9ficos promovidos pelo exerc\u00edcio f\u00edsico na DP com foco nos sintomas motores, deixando de lado os eventos celulares e moleculares que podem estar correlacionados com a melhoria da dor cr\u00f4nica ap\u00f3s essa interven\u00e7\u00e3o n\u00e3o farmacol\u00f3gica. A fisioterapia e o treinamento f\u00edsico t\u00eam se mostrado eficazes na melhoria da qualidade de vida de pacientes diagnosticados com DP que relatam dor. Embora estudos anteriores em pacientes com DP descrevam uma correla\u00e7\u00e3o entre o limiar de dor e a ativa\u00e7\u00e3o de diferentes \u00e1reas no c\u00e9rebro, pouco se sabe sobre os mecanismos envolvidos na melhoria da dor promovida pelo exerc\u00edcio f\u00edsico na DP descrita em estudos cl\u00ednicos. Portanto, o presente estudo colaborativo torna-se importante para avaliar \u00e1reas do SNC, al\u00e9m do caminho, \u00e1reas que podem ser moduladas<\/span> diretamente ou indiretamente no processamento nociceptivo no modelo animal de DP, como a PAG e o c\u00f3rtex cingulado anterior. Este \u00e9 um trabalho colaborativo entre o Instituto de Ci\u00eancias Biom\u00e9dicas e o Instituto de Medicina Cl\u00ednica da Universidade de Aarhus, Dinamarca, com a Dra. Professora Anne M. Landau.<\/span><\/span><\/p>\n

5. Fotobiomodula\u00e7\u00e3o transcraniana como terapia para pacientes com doen\u00e7a de Parkinson<\/b>: Rela\u00e7\u00e3o entre dor e conectividade funcional cerebral: A doen\u00e7a de Parkinson \u00e9 uma doen\u00e7a neurol\u00f3gica progressiva e degenerativa que afeta milhares de pessoas. A doen\u00e7a \u00e9 caracterizada pela apresenta\u00e7\u00e3o de sintomas motores e n\u00e3o motores, e \u00e0 medida que a doen\u00e7a avan\u00e7a, torna-se mais incapacitante, tornando imposs\u00edvel para o indiv\u00edduo realizar tarefas simples. Um sintoma n\u00e3o motor cada vez mais relatado por pacientes e subtratado na pr\u00e1tica cl\u00ednica \u00e9 a dor. Nas \u00faltimas d\u00e9cadas, os poss\u00edveis substratos neurais da dor foram estudados extensivamente, resultando em uma rede potencial de \u00e1reas cerebrais conectadas que se acredita estarem envolvidas no processamento e experi\u00eancia da dor. N\u00e3o h\u00e1 um consenso definitivo sobre todas as \u00e1reas envolvidas em tal rede de dor; no entanto, regi\u00f5es relacionadas \u00e0 dor consistentemente encontradas em todos os estudos incluem o t\u00e1lamo, c\u00f3rtex cingulado anterior (ACC), \u00ednsula posterior e anterior, am\u00edgdala, c\u00f3rtex pr\u00e9-frontal (PFC), c\u00f3rtex somatossensorial secund\u00e1rio (IBS) e subst\u00e2ncia cinzenta periaquedutal (PAG). Com o objetivo de ajudar a melhorar a condi\u00e7\u00e3o dolorosa, terapias n\u00e3o farmacol\u00f3gicas t\u00eam sido estudadas, e uma delas \u00e9 a fototerapia, um m\u00e9todo n\u00e3o invasivo usado por v\u00e1rias \u00e1reas da sa\u00fade, que tem se mostrado cada vez mais eficaz no tratamento da sensibilidade \u00e0 dor diminu\u00edda. O presente estudo tem como objetivo avaliar os efeitos da fotobiomodula\u00e7\u00e3o transcraniana em pacientes com doen\u00e7a de Parkinson. Este \u00e9 um estudo randomizado, no qual pretendemos analisar o efeito da FBM no controle da dor e nas imagens de resson\u00e2ncia magn\u00e9tica. Este \u00e9 um trabalho colaborativo com o Instituto de Ci\u00eancias Biom\u00e9dicas da Universidade de Maryland; Dra. Joyce Teixeira, InRad-FMUSP; Dra. Maria da Gra\u00e7a Martin e Prof. Dr. Hazem Adel Ashmawi.\u00a0<\/span><\/span><\/p>\n

6.<\/strong> <\/span>Associa\u00e7\u00e3o de fotobiomodula\u00e7\u00e3o e exerc\u00edcio de nata\u00e7\u00e3o em ratos com dor neurop\u00e1tica:<\/b> Na pr\u00e1tica cl\u00ednica, tem sido amplamente relatado que a dor neurop\u00e1tica \u00e9 dif\u00edcil de tratar, al\u00e9m disso, a dor neurop\u00e1tica tem sido apontada como um importante problema de sa\u00fade p\u00fablica. As op\u00e7\u00f5es terap\u00eauticas para o manejo da dor neurop\u00e1tica t\u00eam aumentado ao longo dos anos, no entanto, a resposta desses pacientes ainda n\u00e3o \u00e9 satisfat\u00f3ria. Nos \u00faltimos anos, v\u00e1rios estudos t\u00eam mostrado que tratamentos n\u00e3o farmacol\u00f3gicos, como fotobiomodula\u00e7\u00e3o e nata\u00e7\u00e3o, t\u00eam sido cada vez mais utilizados como complementos \u00e0 pr\u00e1tica cl\u00ednica. O objetivo deste trabalho \u00e9 avaliar se a nata\u00e7\u00e3o associada ou n\u00e3o \u00e0 fotobiomodula\u00e7\u00e3o tem seus efeitos potencializados em um modelo de les\u00e3o cr\u00f4nica por constri\u00e7\u00e3o. Uma investiga\u00e7\u00e3o de alternativas terap\u00eauticas para o tratamento da dor neurop\u00e1tica \u00e9 importante, uma vez que essa patologia n\u00e3o responde satisfatoriamente a nenhum tipo de interven\u00e7\u00e3o convencional. Nossos resultados demonstraram um efeito ben\u00e9fico na dor e na resposta motora quando associamos ambas as terapias.<\/span><\/span><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Contribution to Science 1. Effect of photobiomodulation on different peripheral neuropathies in rats:\u00a0 The technique of photobiomodulation (PBMT), use of light as therapy, is a non-invasive method that has been shown both in basic and in clinical research to be effective in reducing pain sensitivity and consequently improving the quality of life of patients with[…]<\/a><\/p>\n","protected":false},"author":616,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-190","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/land.icb.usp.br\/en\/wp-json\/wp\/v2\/pages\/190","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/land.icb.usp.br\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/land.icb.usp.br\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/land.icb.usp.br\/en\/wp-json\/wp\/v2\/users\/616"}],"replies":[{"embeddable":true,"href":"https:\/\/land.icb.usp.br\/en\/wp-json\/wp\/v2\/comments?post=190"}],"version-history":[{"count":5,"href":"https:\/\/land.icb.usp.br\/en\/wp-json\/wp\/v2\/pages\/190\/revisions"}],"predecessor-version":[{"id":1455,"href":"https:\/\/land.icb.usp.br\/en\/wp-json\/wp\/v2\/pages\/190\/revisions\/1455"}],"wp:attachment":[{"href":"https:\/\/land.icb.usp.br\/en\/wp-json\/wp\/v2\/media?parent=190"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}